More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-TRIM28 Antibody
Anti-TRIM28 Antibody

Anti-TRIM28 Antibody

A35695
Antibodies.com
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-TRIM28 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Mouse monoclonal antibody to TRIM28
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-TRIM28 Antibody144-02245
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetTRIM28
  • SizePrice
Product group Antibodies
Biomatik
Trim28 Polyclonal AntibodyCAC07189
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
TargetTRIM28
  • SizePrice
Figure 1. Western blot analysis of KAP1 using anti-KAP1 antibody (PB9835). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAP1 antigen affinity purified polyclonal antibody (Catalog # PB9835) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAP1 at approximately 100 kDa. The expected band size for KAP1 is at 89 kDa.
Product group Antibodies
Boster Bio
Anti-KAP1/TRIM28 Antibody Picoband(r)PB9835-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetTRIM28
  • SizePrice
Product group Antibodies
Assay Genie
KAP1/TRIM28 Monoclonal AntibodyCAB19568
TargetTRIM28
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-TRIM28 [RAB-C429]AB01909-1.1-BT
ApplicationsImmunoFluorescence, ImmunoPrecipitation
TargetTRIM28
  • SizePrice
Human rectal carcinoma lysates probed with Anti Phospho-TIF1 beta(Ser824) Polyclonal Antibody, Unconjugated (bs-3423R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 41kD. Observed band size:41kD.
Product group Antibodies
References
Bioss Antibodies
Phospho-TRIM28 (Ser824) Polyclonal AntibodyBS-3423R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetTRIM28
  • SizePrice
Western Blot Positive WB detected in: PC-3 whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate All lanes: TRIM28 antibody at 1.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 89, 80 kDa Observed band size: 89 kDa
Product group Antibodies
Cusabio
TRIM28 AntibodyCSB-PA05254A0RB
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTRIM28
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
TRIM28 / KAP1 AntibodyLS-C332012
ApplicationsWestern Blot
TargetTRIM28
  • SizePrice
Immunohistochemical staining of human tonsil shows strong nuclear positivity in germinal center cells and non-germinal center cells.
Product group Antibodies
Atlas Antibodies
Anti-TRIM28 AntibodyHPA064033
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTRIM28
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-KAP-1 Antibody, Affinity PurifiedA300-274A
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetTRIM28
  • SizePrice