More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Phospho-TRIM28 (Ser824) Polyclonal Antibody
Human rectal carcinoma lysates probed with Anti Phospho-TIF1 beta(Ser824) Polyclonal Antibody, Unconjugated (bs-3423R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 41kD. Observed band size:41kD.
Human rectal carcinoma lysates probed with Anti Phospho-TIF1 beta(Ser824) Polyclonal Antibody, Unconjugated (bs-3423R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 41kD. Observed band size:41kD.
Human rectal carcinoma lysates probed with Anti Phospho-TIF1 beta(Ser824) Polyclonal Antibody, Unconjugated (bs-3423R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 41kD. Observed band size:41kD.

Phospho-TRIM28 (Ser824) Polyclonal Antibody

BS-3423R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTRIM28
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    Phospho-TRIM28 (Ser824) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID10155
  • Target name
    TRIM28
  • Target description
    tripartite motif containing 28
  • Target synonyms
    KAP1, PPP1R157, RNF96, TF1B, TIF1B, TIF1beta, WT7, transcription intermediary factor 1-beta, E3 SUMO-protein ligase TRIM28, KAP-1, KRAB [Kruppel-associated box domain]-associated protein 1, KRAB-interacting protein 1, KRIP-1, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, TIF1-beta, nuclear corepressor KAP-1, protein phosphatase 1, regulatory subunit 157, transcriptional intermediary factor 1-beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13263
  • Protein Name
    Transcription intermediary factor 1-beta
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Ding D, Zhang Y, Wang J, et al. γ-H2AX/53BP1/pKAP-1 foci and their linear tracks induced by in vitro exposure to radon and its progeny in human peripheral blood lymphocytes. Sci Rep. 2016,6:38295. doi: 10.1038/srep38295
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot Positive WB detected in: PC-3 whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate All lanes: TRIM28 antibody at 1.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 89, 80 kDa Observed band size: 89 kDa
Product group Antibodies
Cusabio
TRIM28 AntibodyCSB-PA05254A0RB
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTRIM28
  • SizePrice
Product group Antibodies
MedChem Express
KAP1 Antibody (YA3523, Rabbit)HY-P80730
TargetTRIM28
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-TRIM28 [RAB-C429]AB01909-1.1-BT
ApplicationsImmunoFluorescence, ImmunoPrecipitation
TargetTRIM28
  • SizePrice
Figure 1. Western blot analysis of KAP1 using anti-KAP1 antibody (PB9835). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAP1 antigen affinity purified polyclonal antibody (Catalog # PB9835) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAP1 at approximately 100 kDa. The expected band size for KAP1 is at 89 kDa.
Product group Antibodies
Boster Bio
Anti-KAP1/TRIM28 Antibody Picoband(r)PB9835-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetTRIM28
  • SizePrice
Product group Antibodies
RayBiotech
Anti-TRIM28 Antibody144-02245
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetTRIM28
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-KAP-1 Antibody, Affinity PurifiedA300-274A
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetTRIM28
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
TRIM28 / KAP1 AntibodyLS-C332012
ApplicationsWestern Blot
TargetTRIM28
  • SizePrice
Anti-TRIM28 Antibody
Product group Antibodies
Antibodies.com
Anti-TRIM28 AntibodyA35695
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • SizePrice
Immunohistochemical staining of human tonsil shows strong nuclear positivity in germinal center cells and non-germinal center cells.
Product group Antibodies
Atlas Antibodies
Anti-TRIM28 AntibodyHPA064033
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTRIM28
  • SizePrice
Product group Antibodies
Assay Genie
KAP1/TRIM28 Monoclonal AntibodyCAB19568
TargetTRIM28
  • SizePrice