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Immunohistochemical staining of human spleen shows strong nuclear and cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows strong nuclear and cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows strong nuclear and cytoplasmic positivity in cells in red pulp.

Anti-TRIM29 Antibody

HPA074841
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRIM29
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRIM29
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23650
  • Target name
    TRIM29
  • Target description
    tripartite motif containing 29
  • Target synonyms
    ATDC, tripartite motif-containing protein 29, ataxia-telangiectasia group D-associated protein, tripartite motif protein TRIM29
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14134
  • Protein Name
    Tripartite motif-containing protein 29
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRIM29 using anti-TRIM29 antibody (A04472-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM29 antigen affinity purified polyclonal antibody (Catalog # A04472-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM29 at approximately 70 kDa. The expected band size for TRIM29 is at 67 kDa.
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