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ProductsBack/TRIM29 (acetyl K116) Polyclonal Antibody

TRIM29 (acetyl K116) Polyclonal Antibody

BS-16731R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTRIM29
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Overview

  • Supplier
    Bioss
  • Product Name
    TRIM29 (acetyl K116) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(ELISA=1:5000-10000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500), ICC/IF(ICC/IF=1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID23650
  • Target name
    TRIM29
  • Target description
    tripartite motif containing 29
  • Target synonyms
    ATDC, tripartite motif-containing protein 29, ataxia-telangiectasia group D-associated protein, tripartite motif protein TRIM29
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14134
  • Protein Name
    Tripartite motif-containing protein 29
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRIM29 using anti-TRIM29 antibody (A04472-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM29 antigen affinity purified polyclonal antibody (Catalog # A04472-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM29 at approximately 70 kDa. The expected band size for TRIM29 is at 67 kDa.
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