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Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in exocrine cells.
Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in exocrine cells.
Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in exocrine cells.

Anti-TRIM5 Antibody

HPA023422
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRIM5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRIM5
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID85363
  • Target name
    TRIM5
  • Target description
    tripartite motif containing 5
  • Target synonyms
    RNF88, TRIM5alpha, tripartite motif-containing protein 5, RING-type E3 ubiquitin transferase TRIM5, ring finger protein 88, tripartite motif protein TRIM5, tripartite motif-containing protein 5 alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9C035
  • Protein Name
    Tripartite motif-containing protein 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TRIM5 using anti-TRIM5 antibody (A01359-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM5 antigen affinity purified polyclonal antibody (Catalog # A01359-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM5 at approximately 56KD. The expected band size for TRIM5 is at 56KD.
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