Western Blot Positive WB detected in: HepG2 whole cell lysate, HEK293 whole cell lysate, A549 whole cell lysate, Mouse heart tissue All lanes: TRIM5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 47, 41, 38, 32, 30 kDa Observed band size: 70 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, HEK293 whole cell lysate, A549 whole cell lysate, Mouse heart tissue All lanes: TRIM5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 47, 41, 38, 32, 30 kDa Observed band size: 70 kDa
TRIM5 Antibody
CSB-PA874861LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRIM5
Overview
- SupplierCusabio
- Product NameTRIM5 Antibody
- Delivery Days Customer20
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID85363
- Target nameTRIM5
- Target descriptiontripartite motif containing 5
- Target synonymsring finger protein 88; RING-type E3 ubiquitin transferase TRIM5; RNF88; TRIM5alpha; tripartite motif containing 5 transcript variant iota; tripartite motif containing 5 transcript variant kappa; tripartite motif protein TRIM5; tripartite motif-containing protein 5
- HostRabbit
- IsotypeIgG
- Protein IDQ9C035
- Protein NameTripartite motif-containing protein 5
- Scientific DescriptionCapsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates \\\Lys-63\\\-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV), equine infectious anemia virus (EIAV), simian immunodeficiency virus of macaques (SIVmac), feline immunodeficiency virus (FIV), and bovine immunodeficiency virus (BIV) (PubMed:17156811). Plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2 (PubMed:25127057). Also plays a role in autophagy by acting as a selective autophagy receptor which recognizes and targets HIV-1 capsid protein p24 for autophagic destruction (PubMed:25127057).
- ReactivityHuman
- Storage Instruction-20°C or -80°C
- UNSPSC12352203