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Immunohistochemical staining of human parathyroid gland shows membranous positivity in glandular cells.
Immunohistochemical staining of human parathyroid gland shows membranous positivity in glandular cells.
Immunohistochemical staining of human parathyroid gland shows membranous positivity in glandular cells.

Anti-TRMT2B Antibody

HPA035120
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRMT2B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRMT2B Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79979
  • Target name
    TRMT2B
  • Target description
    tRNA methyltransferase 2B
  • Target synonyms
    CXorf34, dJ341D10.3, tRNA (uracil-5-)-methyltransferase homolog B, TRM2 homolog, TRM2 homolog B, TRM2 tRNA methyltransferase 2 homolog B, rRNA (uracil-5-)-methyltransferase TRMT2B, tRNA (uracil(54)-C(5))-methyltransferase homolog, tRNA (uracil-5-)-methyltransferase homolog, tRNA methyltransferase 2 homolog B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96GJ1
  • Protein Name
    tRNA (uracil-5-)-methyltransferase homolog B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TRMT2B using anti-TRMT2B antibody (A16400-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT2B antigen affinity purified polyclonal antibody (Catalog # A16400-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT2B at approximately 47 kDa. The expected band size for TRMT2B is at 56 kDa.
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