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Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Anti-TRMT5 Antibody

HPA000943
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetTRMT5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRMT5
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57570
  • Target name
    TRMT5
  • Target description
    tRNA methyltransferase 5
  • Target synonyms
    COXPD26; KIAA1393; M1G-methyltransferase; TRM5; TRM5 tRNA methyltransferase 5 homolog; tRNA (guanine(37)-N1)-methyltransferase; tRNA (guanine-N(1)-)-methyltransferase; tRNA [GM37] methyltransferase; tRNA-N1G37 methyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ32P41
  • Protein Name
    tRNA (guanine(37)-N1)-methyltransferase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRMT5 using anti-TRMT5 antibody (A11359-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT5 antigen affinity purified polyclonal antibody (Catalog # A11359-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT5 at approximately 50 kDa. The expected band size for TRMT5 is at 58 kDa.
Product group Antibodies
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Anti-TRMT5 Antibody Picoband(r)A11359-1-CARRIER-FREE
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WB analysis of MCF-7 cells using GTX46177 TRMT5 antibody at 0.2-1microg/ml.
Product group Antibodies
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TRMT5 antibody, InternalGTX46177
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