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TRMT5 Antibody

LS-C752218
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTRMT5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TRMT5 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57570
  • Target name
    TRMT5
  • Target description
    tRNA methyltransferase 5
  • Target synonyms
    COXPD26; KIAA1393; M1G-methyltransferase; TRM5; TRM5 tRNA methyltransferase 5 homolog; tRNA (guanine(37)-N1)-methyltransferase; tRNA (guanine-N(1)-)-methyltransferase; tRNA [GM37] methyltransferase; tRNA-N1G37 methyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRMT5 using anti-TRMT5 antibody (A11359-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT5 antigen affinity purified polyclonal antibody (Catalog # A11359-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT5 at approximately 50 kDa. The expected band size for TRMT5 is at 58 kDa.
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