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Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Anti-TRMT6 Antibody

HPA047032
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRMT6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRMT6 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51605
  • Target name
    TRMT6
  • Target description
    tRNA methyltransferase 6 non-catalytic subunit
  • Target synonyms
    CGI-09, GCD10, Gcd10p, TRM6, tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, tRNA methyltransferase 6 homolog, tRNA(m1A58)MTase subunit TRM6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UJA5
  • Protein Name
    tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TRMT6 using anti-TRMT6 antibody (A13158). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human MOLT-4 whole cell lysates, Lane 8: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT6 antigen affinity purified polyclonal antibody (Catalog # A13158) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT6 at approximately 60 kDa. The expected band size for TRMT6 is at 56 kDa.
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