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Western blot All lanes: TRMT6 antibody at 4ug/ml Lane 1: U251 whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 45, 41, 37 kDa Observed band size: 56 kDa
Western blot All lanes: TRMT6 antibody at 4ug/ml Lane 1: U251 whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 45, 41, 37 kDa Observed band size: 56 kDa
Western blot All lanes: TRMT6 antibody at 4ug/ml Lane 1: U251 whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 45, 41, 37 kDa Observed band size: 56 kDa

TRMT6 Antibody

CSB-PA891716LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRMT6
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Overview

  • Supplier
    Cusabio
  • Product Name
    TRMT6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51605
  • Target name
    TRMT6
  • Target description
    tRNA methyltransferase 6 non-catalytic subunit
  • Target synonyms
    CGI-09, GCD10, Gcd10p, TRM6, tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, tRNA methyltransferase 6 homolog, tRNA(m1A58)MTase subunit TRM6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UJA5
  • Protein Name
    tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
  • Scientific Description
    Substrate-binding subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRMT6 using anti-TRMT6 antibody (A13158). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human MOLT-4 whole cell lysates, Lane 8: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT6 antigen affinity purified polyclonal antibody (Catalog # A13158) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT6 at approximately 60 kDa. The expected band size for TRMT6 is at 56 kDa.
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