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Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.

Anti-TRNT1 Antibody

HPA036938
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetTRNT1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRNT1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51095
  • Target name
    TRNT1
  • Target description
    tRNA nucleotidyl transferase 1
  • Target synonyms
    CCA1, CGI-47, MtCCA, RPEM, SIFD, CCA tRNA nucleotidyltransferase 1, mitochondrial, ATP(CTP):tRNA nucleotidyltransferase, CCA-adding enzyme, mitochondrial CCA-adding tRNA-nucleotidyltransferase, mt CCA-adding enzyme, mt tRNA CCA-diphosphorylase, mt tRNA CCA-pyrophosphorylase, mt tRNA adenylyltransferase, tRNA CCA nucleotidyl transferase 1, tRNA nucleotidyl transferase, CCA-adding, 1, tRNA-nucleotidyltransferase 1, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96Q11
  • Protein Name
    CCA tRNA nucleotidyltransferase 1, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TRNT1 using anti-TRNT1 antibody (A06030-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRNT1 antigen affinity purified polyclonal antibody (Catalog # A06030-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRNT1 at approximately 50 kDa. The expected band size for TRNT1 is at 50 kDa.
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