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Western Blot Positive WB detected in: 293T whole cell lysate, HepG2 whole cell lysate All lanes: TRNT1 antibody at 5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 48, 7 kDa Observed band size: 51, 48 kDa
Western Blot Positive WB detected in: 293T whole cell lysate, HepG2 whole cell lysate All lanes: TRNT1 antibody at 5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 48, 7 kDa Observed band size: 51, 48 kDa
Western Blot Positive WB detected in: 293T whole cell lysate, HepG2 whole cell lysate All lanes: TRNT1 antibody at 5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 48, 7 kDa Observed band size: 51, 48 kDa

TRNT1 Antibody

CSB-PA836282LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTRNT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TRNT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51095
  • Target name
    TRNT1
  • Target description
    tRNA nucleotidyl transferase 1
  • Target synonyms
    CCA1, CGI-47, MtCCA, RPEM, SIFD, CCA tRNA nucleotidyltransferase 1, mitochondrial, ATP(CTP):tRNA nucleotidyltransferase, CCA-adding enzyme, mitochondrial CCA-adding tRNA-nucleotidyltransferase, mt CCA-adding enzyme, mt tRNA CCA-diphosphorylase, mt tRNA CCA-pyrophosphorylase, mt tRNA adenylyltransferase, tRNA CCA nucleotidyl transferase 1, tRNA nucleotidyl transferase, CCA-adding, 1, tRNA-nucleotidyltransferase 1, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96Q11
  • Protein Name
    CCA tRNA nucleotidyltransferase 1, mitochondrial
  • Scientific Description
    Isoform 1: Adds and repairs the conserved 3-CCA sequence necessary for the attachment of amino acids to the 3 terminus of tRNA molecules, using CTP and ATP as substrates.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of TRNT1 using anti-TRNT1 antibody (A06030-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRNT1 antigen affinity purified polyclonal antibody (Catalog # A06030-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRNT1 at approximately 50 kDa. The expected band size for TRNT1 is at 50 kDa.
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