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ProductsBack/anti-TRPC7 antibody [S64A-36]

anti-TRPC7 antibody [S64A-36]

ARG22233
Arigo Biolaboratories
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetTRPC7
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-TRPC7 antibody [S64A-36]
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    S64A-36
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID57113
  • Target name
    TRPC7
  • Target description
    transient receptor potential cation channel subfamily C member 7
  • Target synonyms
    TRP7, short transient receptor potential channel 7, likley ortholog of mouse transient receptor potential cation channel, subfamily C, member 7, putative capacitative calcium channel, transient receptor protein 7
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRP 7/TRPC7 using anti-TRP 7/TRPC7 antibody (A04658-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human SK-N-SH whole cell lysates, Lane 5: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRP 7/TRPC7 antigen affinity purified polyclonal antibody (Catalog # A04658-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRP 7/TRPC7 at approximately 100 kDa. The expected band size for TRP 7/TRPC7 is at 100 kDa.
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