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ProductsBack/anti-TRPS1, Rabbit Monoclonal (RM518)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human breast carcinoma tissue sections (ER+, PR+, Her2+; Stage IIA) using anti-TRPS1 Rabbit Monoclonal Antibody (Clone RM518) at 1:100 dilution, for 1 hr at room temperature.
Immunohistochemical staining of formalin-fixed and paraffin-embedded human breast carcinoma tissue sections (ER+, PR+, Her2+; Stage IIA) using anti-TRPS1 Rabbit Monoclonal Antibody (Clone RM518) at 1:100 dilution, for 1 hr at room temperature.
Immunohistochemical staining of formalin-fixed and paraffin-embedded human breast carcinoma tissue sections (ER+, PR+, Her2+; Stage IIA) using anti-TRPS1 Rabbit Monoclonal Antibody (Clone RM518) at 1:100 dilution, for 1 hr at room temperature.

anti-TRPS1, Rabbit Monoclonal (RM518)

REV-31-1410-00
RevMAb Biosciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetTRPS1
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Overview

  • Supplier
    RevMAb Biosciences
  • Product Name
    anti-TRPS1, Rabbit Monoclonal (RM518)
  • Delivery Days Customer
    10
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RM518
  • Gene ID7227
  • Target name
    TRPS1
  • Target description
    transcriptional repressor GATA binding 1
  • Target synonyms
    GC79; LGCR; trichorhinophalangeal syndrome I; tricho-rhino-phalangeal syndrome type I protein; zinc finger protein GC79; zinc finger transcription factor Trps1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UHF7
  • Protein Name
    Zinc finger transcription factor Trps1
  • Scientific Description
    Recombinant Antibody. This antibody reacts to human TRPS1). It may also react to mouse or rat TRPS1, as predicted by immunogen homology. Isotype: Rabbit IgG. Immunogen: A recombinant protein corresponding to human TRPS1. Applications: IHC, WB. TRPS1 is a member of the zinc finger family of transcription factors and represses GATA-regulated genes and binds to a dynein light chain protein. Binding of the encoded protein to the dynein light chain protein affects binding to GATA consensus sequences and suppresses its transcriptional activity. Defects in this gene are a cause of tricho-rhino-phalangeal syndrome (TRPS) types I-III. - TRPS1 is a member of the zinc finger family of transcription factors and represses GATA-regulated genes and binds to a dynein light chain protein. Binding of the encoded protein to the dynein light chain protein affects binding to GATA consensus sequences and suppresses its transcriptional activity. Defects in this gene are a cause of tricho-rhino-phalangeal syndrome (TRPS) types I-III.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRPS1 using anti-TRPS1 antibody (A02328-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPS1 antigen affinity purified polyclonal antibody (Catalog # A02328-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPS1 at approximately 150 kDa. The expected band size for TRPS1 is at 142 kDa.
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