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Anti-TSPY2 (Center) Antibody

102-23936
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetTSPY1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-TSPY2 (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB49775
  • Gene ID7258
  • Target name
    TSPY1
  • Target description
    testis specific protein Y-linked 1
  • Target synonyms
    CT78, DYS14, TSPY, pJA923, testis-specific Y-encoded protein 1, cancer/testis antigen 78, testis specific protein, Y-linked
  • Host
    Rabbit
  • Protein IDA6NKD2
  • Protein Name
    Testis-specific Y-encoded protein 2
  • Scientific Description
    Rabbit Anti-TSPY2 (Center) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TSPY1/2/3/4 using anti-TSPY1/2/3/4 antibody (A04215-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSPY1/2/3/4 antigen affinity purified polyclonal antibody (Catalog # A04215-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSPY1/2/3/4 at approximately 39 kDa. The expected band size for TSPY1/2/3/4 is at 35 kDa.
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