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The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA696394(TSPY1/TSPY3 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA696394(TSPY1/TSPY3 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA696394(TSPY1/TSPY3 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: x200)

TSPY1/TSPY3 Antibody

CSB-PA696394
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTSPY1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TSPY1/TSPY3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7258
  • Target name
    TSPY1
  • Target description
    testis specific protein Y-linked 1
  • Target synonyms
    cancer/testis antigen 78; CT78; DYS14; pJA923; testis specific protein, Y-linked; testis-specific Y-encoded protein 1; TSPY
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP0CV98
  • Protein Name
    Testis-specific Y-encoded protein 3
  • Scientific Description
    The protein encoded by this gene is found only in testicular tissue and may be involved in spermatogenesis. Approximately 35 copies of this gene are present in humans, but only a single, nonfunctional orthologous gene is found in mouse. Two transcript variants encoding different isoforms have been found for this gene.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TSPY1/2/3/4 using anti-TSPY1/2/3/4 antibody (A04215-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSPY1/2/3/4 antigen affinity purified polyclonal antibody (Catalog # A04215-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSPY1/2/3/4 at approximately 39 kDa. The expected band size for TSPY1/2/3/4 is at 35 kDa.
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