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Immunofluorescent staining of human cell line HaCaT shows localization to cell junctions.
Immunofluorescent staining of human cell line HaCaT shows localization to cell junctions.
Immunofluorescent staining of human cell line HaCaT shows localization to cell junctions.

Anti-TSSK4 Antibody

HPA060660
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetTSSK4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TSSK4 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID283629
  • Target name
    TSSK4
  • Target description
    testis specific serine kinase 4
  • Target synonyms
    C14orf20, STK22E, TSK-4, TSK4, TSSK5, testis-specific serine/threonine-protein kinase 4, serine/threonine kinase 22E, serine/threonine-protein kinase 22E
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6SA08
  • Protein Name
    Testis-specific serine/threonine-protein kinase 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TSSK4 using anti-TSSK4 antibody (A10441-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSSK4 antigen affinity purified polyclonal antibody (Catalog # A10441-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSSK4 at approximately 42 kDa. The expected band size for TSSK4 is at 37 kDa.
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