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Western Blot analysis of HT29 cells using TSSK 4 Polyclonal Antibody
Western Blot analysis of HT29 cells using TSSK 4 Polyclonal Antibody
Western Blot analysis of HT29 cells using TSSK 4 Polyclonal Antibody

TSSK4 Antibody

CSB-PA004341
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetTSSK4
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Overview

  • Supplier
    Cusabio
  • Product Name
    TSSK4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID283629
  • Target name
    TSSK4
  • Target description
    testis specific serine kinase 4
  • Target synonyms
    C14orf20, STK22E, TSK-4, TSK4, TSSK5, testis-specific serine/threonine-protein kinase 4, serine/threonine kinase 22E, serine/threonine-protein kinase 22E
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6SA08
  • Protein Name
    Testis-specific serine/threonine-protein kinase 4
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TSSK4 using anti-TSSK4 antibody (A10441-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSSK4 antigen affinity purified polyclonal antibody (Catalog # A10441-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSSK4 at approximately 42 kDa. The expected band size for TSSK4 is at 37 kDa.
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