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Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum.

Anti-TTC39B Antibody

HPA063162
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetTTC39B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TTC39B Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID158219
  • Target name
    TTC39B
  • Target description
    tetratricopeptide repeat domain 39B
  • Target synonyms
    C9orf52, tetratricopeptide repeat protein 39B, TPR repeat protein 39B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5VTQ0
  • Protein Name
    Tetratricopeptide repeat protein 39B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TTC39B using anti-TTC39B antibody (A13852-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC39B antigen affinity purified polyclonal antibody (Catalog # A13852-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC39B at approximately 70 kDa. The expected band size for TTC39B is at 70 kDa.
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Anti-TTC39B Antibody Picoband(r)A13852-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetTTC39B
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Protein IDQ5VTQ0
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Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Product group Antibodies
Atlas Antibodies
Anti-TTC39B AntibodyHPA054804
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Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Product group Antibodies
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ReactivityHuman
TargetTTC39B
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Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate All lanes: TTC39B antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 77, 67, 76, 60, 70, 23 kDa Observed band size: 77 kDa
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TargetTTC39B
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Sample (5 ug of whole cell lysate) A: Non-transfected 293T lysates B: TTC39B transfected 293T lysates 7.5% SDS PAGE GTX125938 diluted at 1:10000
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TTC39B antibodyGTX125938
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetTTC39B
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