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Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity.
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity.
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity.

Anti-TTI2 Antibody

HPA023523
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTTI2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TTI2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80185
  • Target name
    TTI2
  • Target description
    TELO2 interacting protein 2
  • Target synonyms
    C8orf41, MRT39, TELO2-interacting protein 2, Tel2 interacting protein 2 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6NXR4
  • Protein Name
    TELO2-interacting protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TTI2 using anti-TTI2 antibody (A12943-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTI2 antigen affinity purified polyclonal antibody (Catalog # A12943-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTI2 at approximately 60 kDa. The expected band size for TTI2 is at 57 kDa.
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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Rat kidney tissue All lanes: TTI2 antibody at 8.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa
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