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Immunohistochemical staining of human skin shows cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows cytoplasmic positivity in squamous epithelial cells.

Anti-TULP3 Antibody

HPA015285
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetTULP3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TULP3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7289
  • Target name
    TULP3
  • Target description
    TUB like protein 3
  • Target synonyms
    tubby like protein 3; tubby-related protein 3; TUBL3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75386
  • Protein Name
    Tubby-related protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TULP3 using anti-TULP3 antibody (A09384-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TULP3 antigen affinity purified polyclonal antibody (Catalog # A09384-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TULP3 at approximately 60 kDa. The expected band size for TULP3 is at 50 kDa.
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