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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus, nucleoli & plasma membrane.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus, nucleoli & plasma membrane.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus, nucleoli & plasma membrane.

Anti-TULP3 Antibody

HPA018496
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetTULP3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TULP3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7289
  • Target name
    TULP3
  • Target description
    TUB like protein 3
  • Target synonyms
    HRCDF, TUBL3, tubby-related protein 3, tubby like protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75386
  • Protein Name
    Tubby-related protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TULP3 using anti-TULP3 antibody (A09384-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TULP3 antigen affinity purified polyclonal antibody (Catalog # A09384-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TULP3 at approximately 60 kDa. The expected band size for TULP3 is at 50 kDa.
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