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Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.

Anti-TWF1 Antibody

HPA018116
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTWF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TWF1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5756
  • Target name
    TWF1
  • Target description
    twinfilin actin binding protein 1
  • Target synonyms
    A6, PTK9, twinfilin-1, A6 protein tyrosine kinase, PTK9 protein tyrosine kinase 9, protein A6, protein tyrosine kinase 9, twinfilin, actin-binding protein, homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12792
  • Protein Name
    Twinfilin-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TWF1 using anti-TWF1 antibody (A07004-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT-1080 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWF1 antigen affinity purified polyclonal antibody (Catalog # A07004-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWF1 at approximately 42 kDa. The expected band size for TWF1 is at 40,42 kDa.
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