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The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA090875(TWF1 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA090875(TWF1 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA090875(TWF1 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)

TWF1 Antibody

CSB-PA090875
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTWF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TWF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5756
  • Target name
    TWF1
  • Target description
    twinfilin actin binding protein 1
  • Target synonyms
    A6, PTK9, twinfilin-1, A6 protein tyrosine kinase, PTK9 protein tyrosine kinase 9, protein A6, protein tyrosine kinase 9, twinfilin, actin-binding protein, homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12792
  • Protein Name
    Twinfilin-1
  • Scientific Description
    This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TWF1 using anti-TWF1 antibody (A07004-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT-1080 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWF1 antigen affinity purified polyclonal antibody (Catalog # A07004-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWF1 at approximately 42 kDa. The expected band size for TWF1 is at 40,42 kDa.
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