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Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in spermatids.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in spermatids.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in spermatids.

Anti-UBA6 Antibody

HPA037001
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetUBA6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-UBA6 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55236
  • Target name
    UBA6
  • Target description
    ubiquitin like modifier activating enzyme 6
  • Target synonyms
    E1-L2, MOP-4, UBE1L2, ubiquitin-like modifier-activating enzyme 6, UBA6, ubiquitin-activating enzyme E1, monocyte protein 4, ubiquitin-activating enzyme 6, ubiquitin-activating enzyme E1-like 2, ubiquitin-activating enzyme E1-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDA0AVT1
  • Protein Name
    Ubiquitin-like modifier-activating enzyme 6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of UBA6 using anti-UBA6 antibody (A03213-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA6 antigen affinity purified polyclonal antibody (Catalog # A03213-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBA6 at approximately 118 kDa. The expected band size for UBA6 is at 118 kDa.
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