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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA025420ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA025420ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA025420ESR1HU at dilution of 1:100

UBA6 Antibody

CSB-PA025420ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetUBA6
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Overview

  • Supplier
    Cusabio
  • Product Name
    UBA6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55236
  • Target name
    UBA6
  • Target description
    ubiquitin like modifier activating enzyme 6
  • Target synonyms
    E1-L2, MOP-4, UBE1L2, ubiquitin-like modifier-activating enzyme 6, UBA6, ubiquitin-activating enzyme E1, monocyte protein 4, ubiquitin-activating enzyme 6, ubiquitin-activating enzyme E1-like 2, ubiquitin-activating enzyme E1-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDA0AVT1
  • Protein Name
    Ubiquitin-like modifier-activating enzyme 6
  • Scientific Description
    Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of UBA6 using anti-UBA6 antibody (A03213-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA6 antigen affinity purified polyclonal antibody (Catalog # A03213-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBA6 at approximately 118 kDa. The expected band size for UBA6 is at 118 kDa.
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