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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus.

Anti-UBD-25ul

HPA043710
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-UBD-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    ICC, WB
  • Category Supplier
    Antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Gene ID
    O15205
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15205
  • Protein Name
    Ubiquitin D
  • Scientific Description
    Rabbit Polyclonal Anti-UBD Antibody against Human ubiquitin D. Validated for Immunofluorescence and Western Blot
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UBD using anti-UBD antibody (A01970-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBD antigen affinity purified polyclonal antibody (Catalog # A01970-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBD at approximately 18KD. The expected band size for UBD is at 18KD.
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