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Immunohistochemical staining of human cerebellum shows moderate positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate positivity in Purkinje cells.

Anti-UCHL1 Antibody

AMAB91145
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetUCHL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-UCHL1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL3210
  • Conjugate
    Unconjugated
  • Gene ID7345
  • Target name
    UCHL1
  • Target description
    ubiquitin C-terminal hydrolase L1
  • Target synonyms
    epididymis luminal protein 117; epididymis secretory protein Li 53; HEL-117; HEL-S-53; NDGOA; neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; PGP95; SPG79; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin thioesterase L1; ubiquitin thiolesterase; Uch-L1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP09936
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase isozyme L1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PGP9.5 using anti-PGP9.5 antibody (PB9840). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGP9.5 antigen affinity purified polyclonal antibody (Catalog # PB9840) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGP9.5 at approximately 25 kDa. The expected band size for PGP9.5 is at 25 kDa.
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