
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX101093) diluted at 1:1000.
PGP9.5 antibody
GTX101093
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetUCHL1
Overview
- SupplierGeneTex
- Product NamePGP9.5 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:10000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.56 mg/ml
- ConjugateUnconjugated
- Gene ID7345
- Target nameUCHL1
- Target descriptionubiquitin C-terminal hydrolase L1
- Target synonymsHEL-117, HEL-S-53, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, SPG79, SPG79A, UCHL-1, Uch-L1, ubiquitin carboxyl-terminal hydrolase isozyme L1, epididymis luminal protein 117, epididymis secretory protein Li 53, neuron cytoplasmic protein 9.5, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), ubiquitin thioesterase L1, ubiquitin thiolesterase
- HostRabbit
- IsotypeIgG
- Protein IDP09936
- Protein NameUbiquitin carboxyl-terminal hydrolase isozyme L1
- Scientific DescriptionUCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).[supplied by OMIM]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203



![PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: PGP9.5 protein stained by PGP9.5 antibody (GTX101093) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: PGP9.5 protein stained by PGP9.5 antibody (GTX101093) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX101093/GTX101093_39834_20161012_IHC-Fr_R_w_23060100_140.webp)
![IHC-P analysis of human cerebellum tissue section using GTX02737 PGP9.5 antibody [UCHL1/4556R].](https://www.genetex.com/upload/website/prouct_img/normal/GTX02737/GTX02737_20210319_IHC-P_1_w_23053122_123.webp)
![IHC-P analysis of human testis tissue using GTX04438 PGP9.5 antibody [MSVA-905R] HistoMAX?. A strong PGP9.5 immunostaining is seen in spermatogonia. PGP9.5 staining decreases sharply in spermatocytes where it is only faint. A moderate PGP9.5 staining is seen in Leydig cells while Sertoli cells remain PGP9.5 negative.](https://www.genetex.com/upload/website/prouct_img/normal/GTX04438/GTX04438_20230728_IHC-P_97_23072722_457.webp)


![PGP9.5 antibody detects PGP9.5 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109646) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX109646/GTX109646_39959_20170503_IFA_R_w_23060500_628.webp)

![PGP9.5 antibody [GT448] detects PGP9.5 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGP9.5 stained by PGP9.5 antibody [GT448] (GTX634797) diluted at 1:500. Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX634797/GTX634797_43339_20190306_ICC_IF_R_w_23061202_510.webp)