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Anti-ULK3 Antibody

Anti-ULK3 Antibody

A85002
Antibodies.com
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-ULK3 Antibody
  • Delivery Days Customer
    7
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Host
    Goat
  • Isotype
    IgG
  • Scientific Description
    Goat polyclonal antibody to ULK3.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ULK3 using anti-ULK3 antibody (PB9519). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Rat Testis Tissue Lysate at 50ug, Lane 3: Mouse Brain Tissue Lysate at 50ug, Lane 4: Human Placenta Tissue Lysate at 50ug, Lane 5: 22RV1 Whole Cell Lysate at 40ug, Lane 6: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ULK3 antigen affinity purified polyclonal antibody (Catalog # PB9519) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ULK3 at approximately 53 kDa. The expected band size for ULK3 is at 53 kDa.
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References
Everest Biotech
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