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Western Blot analysis of 293 cells using ULK3 Polyclonal Antibody
Western Blot analysis of 293 cells using ULK3 Polyclonal Antibody
Western Blot analysis of 293 cells using ULK3 Polyclonal Antibody

ULK3 Antibody

CSB-PA004389
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetULK3
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Overview

  • Supplier
    Cusabio
  • Product Name
    ULK3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25989
  • Target name
    ULK3
  • Target description
    unc-51 like kinase 3
  • Target synonyms
    serine/threonine-protein kinase ULK3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6PHR2
  • Protein Name
    Serine/threonine-protein kinase ULK3
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ULK3 using anti-ULK3 antibody (PB9519). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Rat Testis Tissue Lysate at 50ug, Lane 3: Mouse Brain Tissue Lysate at 50ug, Lane 4: Human Placenta Tissue Lysate at 50ug, Lane 5: 22RV1 Whole Cell Lysate at 40ug, Lane 6: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ULK3 antigen affinity purified polyclonal antibody (Catalog # PB9519) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ULK3 at approximately 53 kDa. The expected band size for ULK3 is at 53 kDa.
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