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Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Anti-UPF1 Antibody

HPA020857
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetUPF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-UPF1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5976
  • Target name
    UPF1
  • Target description
    UPF1 RNA helicase and ATPase
  • Target synonyms
    HUPF1, NORF1, RENT1, UTF, pNORF1, smg-2, regulator of nonsense transcripts 1, ATP-dependent helicase RENT1, UPF1 regulator of nonsense transcripts homolog, delta helicase, nonsense mRNA reducing factor 1, smg-2 homolog, nonsense mediated mRNA decay factor, up-frameshift mutation 1 homolog, up-frameshift suppressor 1 homolog, yeast Upf1p homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92900
  • Protein Name
    Regulator of nonsense transcripts 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody (PB9842). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: rat RH35 whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RENT1/hUPF1 antigen affinity purified polyclonal antibody (Catalog # PB9842) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RENT1/hUPF1 at approximately 140 kDa. The expected band size for RENT1/hUPF1 is at 124 kDa.
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