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Immunohistochemical staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.

Anti-UQCRH Antibody

HPA042574
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetUQCRH
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-UQCRH Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7388
  • Target name
    UQCRH
  • Target description
    ubiquinol-cytochrome c reductase hinge protein
  • Target synonyms
    MC3DN11, QCR6, UQCR8, cytochrome b-c1 complex subunit 6, mitochondrial, complex III subunit 6, cytochrome c1 non-heme 11 kDa protein, mitochondrial hinge protein, ubiquinol-cytochrome c reductase complex 11 kDa protein, ubiquinol-cytochrome c reductase, complex III subunit VIII
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07919
  • Protein Name
    Cytochrome b-c1 complex subunit 6, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of UQCRH using anti-UQCRH antibody (M09436). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UQCRH antigen affinity purified monoclonal antibody (Catalog # M09436) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UQCRH at approximately 13 kDa. The expected band size for UQCRH is at 11 kDa.
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