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Western blot analysis of USP10 expression in A375 cell lysate.
Western blot analysis of USP10 expression in A375 cell lysate.
Western blot analysis of USP10 expression in A375 cell lysate.

Anti-USP10 Monoclonal Antibody

M03786
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetUSP10
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-USP10 Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ADCB-21
  • Gene ID9100
  • Target name
    USP10
  • Target description
    ubiquitin specific peptidase 10
  • Target synonyms
    deubiquitinating enzyme 10; ubiquitin carboxyl-terminal hydrolase 10; ubiquitin specific protease 10; ubiquitin thioesterase 10; ubiquitin thiolesterase 10; ubiquitin-specific-processing protease 10; UBPO
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14694
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase 10
  • Scientific Description
    Boster Bio Anti-USP10 Monoclonal Antibody catalog # M03786. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, Rat liver tissue All lanes: USP10 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 88, 93 kDa Observed band size: 88 kDa
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Figure 1. Western blot analysis of USP10 using anti-USP10 antibody (A03786-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human SIHA whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP10 antigen affinity purified polyclonal antibody (Catalog # A03786-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP10 at approximately 110,130 kDa. The expected band size for USP10 is at 87 kDa.
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