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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Anti-VAPB Antibody

HPA013144
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVAPB
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-VAPB Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9217
  • Target name
    VAPB
  • Target description
    VAMP associated protein B and C
  • Target synonyms
    ALS8, VAMP-B, VAP-B, vesicle-associated membrane protein-associated protein B/C, VAMP (vesicle-associated membrane protein)-associated protein B and C, VAMP-associated 33 kDa protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95292
  • Protein Name
    Vesicle-associated membrane protein-associated protein B/C
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of VAPB using anti-VAPB antibody (A01372). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human PANC-1 whole cell lysates, Lane 6: human A375 whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAPB antigen affinity purified polyclonal antibody (Catalog # A01372) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VAPB at approximately 27KD. The expected band size for VAPB is at 27KD.
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