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VAPB Antibody

LS-C334013
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetVAPB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    VAPB Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 11kDa/27kDa, while the observed MW by Western blot was 35kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:20 - 1:50), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 11kDa/27kDa, while the observed MW by Western blot was 35kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9217
  • Target name
    VAPB
  • Target description
    VAMP associated protein B and C
  • Target synonyms
    ALS8, VAMP-B, VAP-B, vesicle-associated membrane protein-associated protein B/C, VAMP (vesicle-associated membrane protein)-associated protein B and C, VAMP-associated 33 kDa protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VAPB using anti-VAPB antibody (A01372). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human PANC-1 whole cell lysates, Lane 6: human A375 whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAPB antigen affinity purified polyclonal antibody (Catalog # A01372) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VAPB at approximately 27KD. The expected band size for VAPB is at 27KD.
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