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Anti-VASA protein [1E9]

AB03563-10.0-BT
Absolute Antibody
ApplicationsELISA
Product group Antibodies
TargetDDX4
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-VASA protein [1E9]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The binding characterization of this antibody to human VASA protein and VASA peptides (residues 700-724; residues 712-721; residues 700-709) was done using ELISA. It was found that this antibody was specific for VASA peptide with residues 712-721. The IgG antibody binds human VASA protein in a dose dependent manner with an EC50 of 0.08919 nM (US20180155445).
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1e9
  • Gene ID54514
  • Target name
    DDX4
  • Target description
    DEAD-box helicase 4
  • Target synonyms
    DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD box protein 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; probable ATP-dependent RNA helicase DDX4; VASA; vasa homolog
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDQ9NQI0
  • Protein Name
    Probable ATP-dependent RNA helicase DDX4
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DDX4 using anti-DDX4 antibody (A02448). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX4 antigen affinity purified polyclonal antibody (Catalog # A02448) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX4 at approximately 79 kDa. The expected band size for DDX4 is at 79 kDa.
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