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DDX4 Antibody (YA1208, Rabbit)

HY-P81463
MedChem Express
TargetDDX4
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    DDX4 Antibody (YA1208, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID54514
  • Target name
    DDX4
  • Target description
    DEAD-box helicase 4
  • Target synonyms
    VASA, probable ATP-dependent RNA helicase DDX4, putative ATP-dependent RNA helicase DDX4, DEAD (Asp-Glu-Ala-Asp) box polypeptide 4, DEAD box protein 4, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4, vasa homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    DDX4 Antibody (YA1208) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DDX4.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DDX4 using anti-DDX4 antibody (A02448). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX4 antigen affinity purified polyclonal antibody (Catalog # A02448) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX4 at approximately 79 kDa. The expected band size for DDX4 is at 79 kDa.
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