Figure 1. Western blot analysis of VCP using anti-VCP antibody (PB9454). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human SH-SY5Y whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human Raji whole cell lysates, Lane 8: human HepG2 whole cell lysates, Lane 9: rat heart tissue lysates, Lane 10: rat spleen tissue lysates, Lane 11: rat kidney tissue lysates, Lane 12: rat liver tissue lysates, Lane 13: mouse heart tissue lysates, Lane 14: mouse kidney tissue lysates, Lane 15: mouse liver tissue lysates, Lane 16: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP antigen affinity purified polyclonal antibody (Catalog # PB9454) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCP at approximately 97KD. The expected band size for VCP is at 89KD.
Figure 1. Western blot analysis of VCP using anti-VCP antibody (PB9454). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human SH-SY5Y whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human Raji whole cell lysates, Lane 8: human HepG2 whole cell lysates, Lane 9: rat heart tissue lysates, Lane 10: rat spleen tissue lysates, Lane 11: rat kidney tissue lysates, Lane 12: rat liver tissue lysates, Lane 13: mouse heart tissue lysates, Lane 14: mouse kidney tissue lysates, Lane 15: mouse liver tissue lysates, Lane 16: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP antigen affinity purified polyclonal antibody (Catalog # PB9454) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCP at approximately 97KD. The expected band size for VCP is at 89KD.
Anti-VCP Antibody Picoband(r)
PB9454-CY3
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetVCP
Overview
- SupplierBoster Bio
- Product NameAnti-VCP Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for VCP is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateCy3
- Gene ID7415
- Target nameVCP
- Target descriptionvalosin containing protein
- Target synonyms15S Mg(2+)-ATPase p97 subunit; CDC48; FTDALS6; p97; TER ATPase; TERA; transitional endoplasmic reticulum ATPase
- HostRabbit
- IsotypeIgG
- Protein IDP55072
- Protein NameTransitional endoplasmic reticulum ATPase
- Scientific DescriptionBoster Bio Anti-VCP Antibody Picoband® catalog # PB9454. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203