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VCP antibody

GTX101089
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetVCP
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Overview

  • Supplier
    GeneTex
  • Product Name
    VCP antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.33 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7415
  • Target name
    VCP
  • Target description
    valosin containing protein
  • Target synonyms
    15S Mg(2+)-ATPase p97 subunit; CDC48; FTDALS6; p97; TER ATPase; TERA; transitional endoplasmic reticulum ATPase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55072
  • Protein Name
    Transitional endoplasmic reticulum ATPase
  • Scientific Description
    The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • An Integrated In Silico, In Vitro and Tumor Tissues Study Identified Selenoprotein S (SELENOS) and Valosin-Containing Protein (VCP/p97) as Novel Potential Associated Prognostic Biomarkers in Triple Negative Breast Cancer. Costantini S et al., 2022 Jan 27, Cancers (Basel)
    Read more
  • Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA. Bozic J et al., 2022 Apr 18, Brain
    Read more
  • Anti-cancer activity of amorphous curcumin preparation in patient-derived colorectal cancer organoids. Elbadawy M et al., 2021 Oct, Biomed Pharmacother
    Read more
  • Perphenazine exerts antitumor effects on HUT78 cells through Akt dephosphorylation by protein phosphatase 2A. Tsuji S et al., 2021 Feb, Oncol Lett
    Read more
  • Identification of Cellular Pathogenicity Markers for SIL1 Mutations Linked to Marinesco-Sjoegren Syndrome. Gatz C et al., 2019, Front Neurol
    Read more
  • PTPN12/PTP-PEST Regulates Phosphorylation-Dependent Ubiquitination and Stability of Focal Adhesion Substrates in Invasive Glioblastoma Cells. Chen Z et al., 2018 Jul 15, Cancer Res
    Read more
  • Endoplasmic Reticulum Protein TXNDC5 Augments Myocardial Fibrosis by Facilitating Extracellular Matrix Protein Folding and Redox-Sensitive Cardiac Fibroblast Activation. Shih YC et al., 2018 Apr 13, Circ Res
    Read more
  • Establishment of a novel three-dimensional primary culture model for hippocampal neurogenesis. Usui T et al., 2017 Jun, Physiol Rep
    Read more
  • RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Inano S et al., 2017 Jun 1, Mol Cell
    Read more
  • Proteomics of muscle chronological ageing in post-menopausal women. Gueugneau M et al., 2014 Dec 23, BMC Genomics
    Read more

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Figure 1. Western blot analysis of VCP using anti-VCP antibody (A00610-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: Monkey COS-7 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human placenta tissue lysates, Lane 8: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP antigen affinity purified polyclonal antibody (Catalog # A00610-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCP at approximately 97 kDa. The expected band size for VCP is at 89 kDa.
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