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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night
Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night
Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-VDAC3 Antibody

A04802-1
Boster Bio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVDAC3
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-VDAC3 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID7419
  • Target name
    VDAC3
  • Target description
    voltage dependent anion channel 3
  • Target synonyms
    HD-VDAC3, VDAC-3, non-selective voltage-gated ion channel VDAC3, outer mitochondrial membrane protein porin 3, voltage-dependent anion-selective channel protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y277
  • Protein Name
    Non-selective voltage-gated ion channel VDAC3
  • Scientific Description
    Boster Bio Anti-VDAC3 Antibody catalog # A04802-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of VDAC3 using anti-VDAC3 antibody (A04802-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VDAC3 antigen affinity purified polyclonal antibody (Catalog # A04802-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VDAC3 at approximately 31KD. The expected band size for VDAC3 is at 31KD.
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