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ProductsBack/Anti-VE-Cadherin CDH5-Antibody Picoband(r)
Anti-VE Cadherin antibody, PA2079, Western blotting All lanes: Anti VE Cadherin (PA2079) at 0.5ug/ml Lane 1: A549 Whole Cell Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Lane 3: MCF-7 Whole Cell Lysate at 40ug Predicted bind size: 87KD Observed bind size: 130KD
Anti-VE Cadherin antibody, PA2079, Western blotting All lanes: Anti VE Cadherin (PA2079) at 0.5ug/ml Lane 1: A549 Whole Cell Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Lane 3: MCF-7 Whole Cell Lysate at 40ug Predicted bind size: 87KD Observed bind size: 130KD
Anti-VE Cadherin antibody, PA2079, Western blotting All lanes: Anti VE Cadherin (PA2079) at 0.5ug/ml Lane 1: A549 Whole Cell Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Lane 3: MCF-7 Whole Cell Lysate at 40ug Predicted bind size: 87KD Observed bind size: 130KD

Anti-VE-Cadherin CDH5-Antibody Picoband(r)

PA2079-IFLUOR647
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDH5
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-VE-Cadherin CDH5-Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Other Conjugate
  • Gene ID1003
  • Target name
    CDH5
  • Target description
    cadherin 5
  • Target synonyms
    7B4, CD144, cadherin-5, 7B4 antigen, VE-cadherin, cadherin 5, type 2 (vascular endothelium), cadherin 5, type 2, VE-cadherin (vascular epithelium), cd144 antigen, endothelial-specific cadherin, vascular endothelial cadherin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP33151
  • Protein Name
    Cadherin-5
  • Scientific Description
    Boster Bio Anti-VE-Cadherin CDH5-Antibody catalog # PA2079. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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HUVEC cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD144/VE Cadherin Polyclonal Antibody(bs-22363R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Figure 1. Western blot analysis of VE Cadherin using anti-VE Cadherin antibody (A02632-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VE Cadherin antigen affinity purified polyclonal antibody (Catalog # A02632-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VE Cadherin at approximately 120 kDa. The expected band size for VE Cadherin is at 88 kDa.
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Boster Bio
Anti-VE-Cadherin CDH5-Antibody Picoband(r)A02632-1-CARRIER-FREE
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ReactivityHuman
TargetCDH5
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