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Immunohistochemical staining of human heart muscle shows strong positivity in intercalated discs.
Immunohistochemical staining of human heart muscle shows strong positivity in intercalated discs.
Immunohistochemical staining of human heart muscle shows strong positivity in intercalated discs.

Anti-VEGFD Antibody

HPA027342
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetVEGFD
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-VEGFD
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2277
  • Target name
    VEGFD
  • Target description
    vascular endothelial growth factor D
  • Target synonyms
    c-fos induced growth factor (vascular endothelial growth factor D); FIGF; vascular endothelial growth factor D; VEGF-D
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43915
  • Protein Name
    Vascular endothelial growth factor D
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VEGFD using anti-VEGFD antibody (PB9869). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFD antigen affinity purified polyclonal antibody (Catalog # PB9869) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGFD at approximately 40KD. The expected band size for VEGFD is at 40KD.
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