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Western Blot analysis of COS7 cells using VEGF-D Polyclonal Antibody
Western Blot analysis of COS7 cells using VEGF-D Polyclonal Antibody
Western Blot analysis of COS7 cells using VEGF-D Polyclonal Antibody

FIGF Antibody

CSB-PA004446
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetVEGFD
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Overview

  • Supplier
    Cusabio
  • Product Name
    FIGF Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2277
  • Target name
    VEGFD
  • Target description
    vascular endothelial growth factor D
  • Target synonyms
    c-fos induced growth factor (vascular endothelial growth factor D); FIGF; vascular endothelial growth factor D; VEGF-D
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43915
  • Protein Name
    Vascular endothelial growth factor D
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of VEGFD using anti-VEGFD antibody (PB9869). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFD antigen affinity purified polyclonal antibody (Catalog # PB9869) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGFD at approximately 40KD. The expected band size for VEGFD is at 40KD.
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