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Anti-VPS4A Antibody

144-66291
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetVPS4A
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-VPS4A Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID27183
  • Target name
    VPS4A
  • Target description
    vacuolar protein sorting 4 homolog A
  • Target synonyms
    CIMDAG, SKD1, SKD1A, SKD2, VPS4, VPS4-1, vacuolar protein sorting-associated protein 4A, SKD1-homolog, hVPS4, vacuolar protein sorting factor 4A, vacuolar sorting protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UN37
  • Protein Name
    Vacuolar protein sorting-associated protein 4A
  • Scientific Description
    VPS4A pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VPS4A using anti-VPS4A antibody (A03216-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS4A antigen affinity purified polyclonal antibody (Catalog # A03216-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPS4A at approximately 50KD. The expected band size for VPS4A is at 50KD.
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