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Anti-VPS4A Antibody

Anti-VPS4A Antibody

A46366
Antibodies.com
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-VPS4A Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.8 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to VPS4A
  • Reactivity
    Human
  • UNSPSC
    12352203

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Western blot All lanes: VPS4Aantibody at 4.28ug/ml Lane 1: Mouse kidney tissue Lane 2: Rat gonadal tissue Lane 3: Mouse brain tissue Lane 4: U87 whole cell lysate Lane 5: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49 kDa Observed band size: 49 kDa
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Figure 1. Western blot analysis of VPS4A using anti-VPS4A antibody (A03216-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS4A antigen affinity purified polyclonal antibody (Catalog # A03216-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPS4A at approximately 50KD. The expected band size for VPS4A is at 50KD.
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