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Immunohistochemical staining of human cerebellum shows strong nuclear and cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong nuclear and cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong nuclear and cytoplasmic positivity in Purkinje cells.

Anti-VPS51 Antibody

HPA039650
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetVPS51
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-VPS51 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID738
  • Target name
    VPS51
  • Target description
    VPS51 subunit of GARP complex
  • Target synonyms
    ANG2, ANG3, C11orf2, C11orf3, FFR, PCH13, vacuolar protein sorting-associated protein 51 homolog, VPS51, GARP complex subunit, another new gene 2 protein, protein fat-free homolog, vacuolar protein sorting 51 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UID3
  • Protein Name
    Vacuolar protein sorting-associated protein 51 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of FFR/VPS51 using anti-FFR/VPS51 antibody (A08518-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FFR/VPS51 antigen affinity purified polyclonal antibody (Catalog # A08518-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FFR/VPS51 at approximately 86 kDa. The expected band size for FFR/VPS51 is at 86 kDa.
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