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ProductsBack/VPS51 Antibody
Western Blot Positive WB detected in: Hela whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate All lanes: VPS51 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 87, 73 kDa Observed band size: 87 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate All lanes: VPS51 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 87, 73 kDa Observed band size: 87 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate All lanes: VPS51 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 87, 73 kDa Observed band size: 87 kDa

VPS51 Antibody

CSB-PA883424LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetVPS51
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Overview

  • Supplier
    Cusabio
  • Product Name
    VPS51 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID738
  • Target name
    VPS51
  • Target description
    VPS51 subunit of GARP complex
  • Target synonyms
    ANG2, ANG3, C11orf2, C11orf3, FFR, PCH13, vacuolar protein sorting-associated protein 51 homolog, VPS51, GARP complex subunit, another new gene 2 protein, protein fat-free homolog, vacuolar protein sorting 51 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UID3
  • Protein Name
    Vacuolar protein sorting-associated protein 51 homolog
  • Scientific Description
    Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. VPS51 participates in retrograde transport of acid hydrolase receptors, likely by promoting tethering and SNARE-dependent fusion of endosome-derived carriers to the TGN (PubMed:20685960). Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane (PubMed:25799061).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FFR/VPS51 using anti-FFR/VPS51 antibody (A08518-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FFR/VPS51 antigen affinity purified polyclonal antibody (Catalog # A08518-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FFR/VPS51 at approximately 86 kDa. The expected band size for FFR/VPS51 is at 86 kDa.
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