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Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.

Anti-WDR33 Antibody

HPA026897
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetWDR33
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WDR33
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55339
  • Target name
    WDR33
  • Target description
    WD repeat domain 33
  • Target synonyms
    NET14; pre-mRNA 3' end processing protein WDR33; WD repeat-containing protein 33; WD repeat-containing protein of 146 kDa; WD repeat-containing protein WDC146; WDC146
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9C0J8
  • Protein Name
    pre-mRNA 3' end processing protein WDR33
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WDR33 using anti-WDR33 antibody (A09589-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR33 antigen affinity purified polyclonal antibody (Catalog # A09589-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR33 at approximately 170-180 kDa. The expected band size for WDR33 is at 146 kDa.
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